Standard Operating Procedure (SOP)

<div dir="ltr" style="text-align: left;" trbidi="on"> <div style="text-align: justify;"> <span style="font-size: large;">The microbiology laboratory is one such environment full of microbial flora. In the bioresearch laboratory, the microbes are separated from its native environment and cultured in an artificially controlled environment. The culture conditions in the bioresearch laboratory are designed to achieve the objective of the experiment. In a practical session, those who are present in the bioresearch laboratory will be in direct contact with the microbes. Those who are having low immunity, allergic will have more chances to get diseased while working with pathogenic microorganisms. </span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span></div> <div style="text-align: justify;"> <span style="font-size: large;">The common hazards in the microbiological laboratory are</span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/hazardous-chemicals.html" target="_blank">Chemical Hazards</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/biosafety-level-bsl.html" target="_blank">Pathogenic Microorganisms</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/electrical-hazards.html" target="_blank">Electrical Hazards</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/mechanical-hazards.html" target="_blank">Mechanical Hazards</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/glassware-handle-with-care.html" target="_blank">Glassware</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/sharp-tools-cutting-blades.html" target="_blank">Sharp Tools</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/fire-hazards.html" target="_blank">Fire Hazards</a></span></li> <li><span style="font-size: large;"><a href="http://www.bioresearchprotocol.com/2018/07/hazardous-radiation.html" target="_blank">Hazardous Radiation</a></span></li> </ol> </div> <div style="text-align: justify;"> <span style="font-size: large;">Safety in Bioresearch laboratory can be assessed by</span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span></div> <div style="text-align: justify;"> <span style="font-size: large;">1. <a href="http://www.bioresearchprotocol.com/2018/07/personal-safety-and-responsibility.html" target="_blank">Personal safety</a> - The working person is solely responsible for his safety. Being a good researcher the person should be attentive in the following</span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;">Dressing</span></li> <li><span style="font-size: large;">Lab coat</span></li> <li><span style="font-size: large;">Shoes</span></li> <li><span style="font-size: large;">Hand gloves</span></li> <li><span style="font-size: large;">Nail</span></li> <li><span style="font-size: large;">Eyewear</span></li> <li><span style="font-size: large;">Face mask</span></li> <li><span style="font-size: large;">Handling of hazardous chemicals</span></li> <li><span style="font-size: large;">Handling of pathogenic microorganisms</span></li> <li><span style="font-size: large;">Other own materials which are required for the practical experiment.</span></li> </ol> </div> <div style="text-align: justify;"> <span style="font-size: large;">2. <a href="http://www.bioresearchprotocol.com/2018/07/good-laboratory-practices-glp.html" target="_blank">Laboratory safety</a> - The main purpose of laboratory safety is to maintain the aseptic environment. Due to more number of working personals, it is very difficult to maintain a pure culture in the general microbiology lab. </span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span> <span style="font-size: large;">Laboratory Safety can be achieved by following good laboratory practices (GLP)</span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;">Surface and Floor cleaning by antiseptic and detergent solutions.</span></li> <li><span style="font-size: large;">Materials and glasswares cleaning by sterilization method and surface sterilization.</span></li> <li><span style="font-size: large;">Biological Safety Cabinets.</span></li> <li><span style="font-size: large;">Disposal and Decontamination of Microbial culture and hazardous chemicals.</span></li> </ol> </div> <div style="text-align: justify;"> <span style="font-size: large;">3. <a href="http://www.bioresearchprotocol.com/2018/07/experimental-safety.html" target="_blank">Experimental Safety</a> - </span><br /> <span style="font-size: large;"><br /></span> <span style="font-size: large;"><br /></span> <span style="font-size: large;">4. <a href="http://www.bioresearchprotocol.com/2018/07/labeling-of-research-materials.html" target="_blank">Labeling</a> - Labeling the materials makes its identification easier. Labeling of media, chemicals, reagents, glassware, cultures  </span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span></div> <div style="text-align: justify;"> <span style="font-size: large;">A label should contain </span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;">Name of the Person</span></li> <li><span style="font-size: large;">Name of the Material</span></li> <li><span style="font-size: large;">Date</span></li> <li><span style="font-size: large;">Nature of the material - Hazardous, Biological, Volatile, Acid, Toxic compound, Carcinogen, Explosive, etc.</span></li> <li><span style="font-size: large;">Preservation and storage - Cultures, chemicals, and reagents</span></li> <li><span style="font-size: large;">Safety handling methods</span></li> </ol> </div> <div style="text-align: justify;"> <span style="font-size: large;">5. <a href="http://www.bioresearchprotocol.com/2018/07/observation-and-record.html" target="_blank">Record and observation</a> - This is the documented proof of the experiment carried in the laboratory.</span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span></div> <div style="text-align: justify;"> <span style="font-size: large;">Observation: It is the rough draft of the experiment carried. It should be prepared based on personal work experience during the working period itself.</span></div> <div style="text-align: justify;"> <span style="font-size: large;"><br /></span></div> <div style="text-align: justify;"> <span style="font-size: large;">An observation of an experiment should contain</span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;">Title of the experiment</span></li> <li><span style="font-size: large;">Date of the experiment</span></li> <li><span style="font-size: large;">Aim or objective</span></li> <li><span style="font-size: large;">Materials & Reagents</span></li> <li><span style="font-size: large;">Principle</span></li> <li><span style="font-size: large;">Rough graphical representation, tables, flowcharts and graphs</span></li> <li><span style="font-size: large;">Procedure</span></li> <li><span style="font-size: large;">Result</span></li> </ol> </div> <div style="text-align: justify;"> <span style="font-size: large;">Record: It is the fair draft of the experiment carried. It should be elaborated on from the observation and reference work. A record should have the following along with the observation notes.</span></div> <div style="text-align: justify;"> <ol> <li><span style="font-size: large;">Introduction</span></li> <li><span style="font-size: large;">History of the experiment</span></li> <li><span style="font-size: large;">Interpretation of the experiment</span></li> <li><span style="font-size: large;">Discussions on the experimental procedures and results obtained</span></li> <li><span style="font-size: large;">Key questions </span></li> </ol> </div> </div>

experimental safety laboratory safety personal safety SOP Standard Operating Procedure (SOP)

The microbiology laboratory is one such environment full of microbial flora. In the bioresearch laboratory, the microbes are separated fro...

Read more »