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Bioinformatics is a field of study that uses computation to extract knowledge from biological data. It includes the collection, storage, retrieval, manipulation and modelling of data for analysis, visualization or prediction through the development of algorithms and software.






Bioinformatics

From Wikipedia, the free encyclopedia

Map of the human X chromosome (from the National Center for Biotechnology Informationwebsite).
Bioinformatics /ËŒbaɪ.oʊˌɪnfÉ™rˈmætɪks/ (About this soundlisten) is an interdisciplinary field that develops methods and software tools for understanding biological data. As an interdisciplinary field of science, bioinformatics combines biologycomputer scienceinformation engineeringmathematics and statistics to analyze and interpret biological data. Bioinformatics has been used for in silico analyses of biological queries using mathematical and statistical techniques.
Bioinformatics includes biological studies that use computer programming as part of their methodology, as well as a specific analysis "pipelines" that are repeatedly used, particularly in the field of genomics. Common uses of bioinformatics include the identification of candidates genes and single nucleotide polymorphisms (SNPs). Often, such identification is made with the aim of better understanding the genetic basis of disease, unique adaptations, desirable properties (esp. in agricultural species), or differences between populations. In a less formal way, bioinformatics also tries to understand the organisational principles within nucleic acid and protein sequences, called proteomics.[1]

Introduction[edit]

Bioinformatics has become an important part of many areas of biology. In experimental molecular biology, bioinformatics techniques such as image and signal processing allow extraction of useful results from large amounts of raw data. In the field of genetics, it aids in sequencing and annotating genomes and their observed mutations. It plays a role in the text mining of biological literature and the development of biological and gene ontologies to organize and query biological data. It also plays a role in the analysis of gene and protein expression and regulation. Bioinformatics tools aid in comparing, analyzing and interpreting of genetic and genomic data and more generally in the understanding of evolutionary aspects of molecular biology. At a more integrative level, it helps analyze and catalogue the biological pathways and networks that are an important part of systems biology. In structural biology, it aids in the simulation and modeling of DNA,[2] RNA,[2][3] proteins[4] as well as biomolecular interactions.[5][6][7][8]

History[edit]

Historically, the term bioinformatics did not mean what it means today. Paulien Hogeweg and Ben Hesper coined it in 1970 to refer to the study of information processes in biotic systems.[9][10][11] This definition placed bioinformatics as a field parallel to biochemistry (the study of chemical processes in biological systems).[9]

Sequences[edit]


Sequences of genetic material are frequently used in bioinformatics and are easier to manage using computers than manually.
Computers became essential in molecular biology when protein sequences became available after Frederick Sanger determined the sequence of insulin in the early 1950s. Comparing multiple sequences manually turned out to be impractical. A pioneer in the field was Margaret Oakley Dayhoff.[12] She compiled one of the first protein sequence databases, initially published as books[13] and pioneered methods of sequence alignment and molecular evolution.[14] Another early contributor to bioinformatics was Elvin A. Kabat, who pioneered biological sequence analysis in 1970 with his comprehensive volumes of antibody sequences released with Tai Te Wu between 1980 and 1991.[15]

Goals[edit]

To study how normal cellular activities are altered in different disease states, the biological data must be combined to form a comprehensive picture of these activities. Therefore, the field of bioinformatics has evolved such that the most pressing task now involves the analysis and interpretation of various types of data. This includes nucleotide and amino acid sequencesprotein domains, and protein structures.[16] The actual process of analyzing and interpreting data is referred to as computational biology. Important sub-disciplines within bioinformatics and computational biology include:
  • Development and implementation of computer programs that enable efficient access to, management and use of, various types of information
  • Development of new algorithms (mathematical formulas) and statistical measures that assess relationships among members of large data sets. For example, there are methods to locate a gene within a sequence, to predict protein structure and/or function, and to cluster protein sequences into families of related sequences.
The primary goal of bioinformatics is to increase the understanding of biological processes. What sets it apart from other approaches, however, is its focus on developing and applying computationally intensive techniques to achieve this goal. Examples include: pattern recognitiondata miningmachine learning algorithms, and visualization. Major research efforts in the field include sequence alignmentgene findinggenome assemblydrug designdrug discoveryprotein structure alignmentprotein structure prediction, prediction of gene expression and protein–protein interactionsgenome-wide association studies, the modeling of evolution and cell division/mitosis.
Bioinformatics now entails the creation and advancement of databases, algorithms, computational and statistical techniques, and theory to solve formal and practical problems arising from the management and analysis of biological data.
Over the past few decades, rapid developments in genomic and other molecular research technologies and developments in information technologies have combined to produce a tremendous amount of information related to molecular biology. Bioinformatics is the name given to these mathematical and computing approaches used to glean understanding of biological processes.
Common activities in bioinformatics include mapping and analyzing DNA and protein sequences, aligning DNA and protein sequences to compare them, and creating and viewing 3-D models of protein structures.

Relation to other fields[edit]

Bioinformatics is a science field that is similar to but distinct from biological computation, while it is often considered synonymous to computational biology. Biological computation uses bioengineering and biology to build biological computers, whereas bioinformatics uses computation to better understand biology. Bioinformatics and computational biology involve the analysis of biological data, particularly DNA, RNA, and protein sequences. The field of bioinformatics experienced explosive growth starting in the mid-1990s, driven largely by the Human Genome Project and by rapid advances in DNA sequencing technology.
Analyzing biological data to produce meaningful information involves writing and running software programs that use algorithms from graph theoryartificial intelligencesoft computingdata miningimage processing, and computer simulation. The algorithms in turn depend on theoretical foundations such as discrete mathematicscontrol theorysystem theoryinformation theory, and statistics.

Sequence analysis[edit]


The sequences of different genes or proteins may be aligned side-by-side to measure their similarity. This alignment compares protein sequences and genomic sequences containing WPP domains.
Since the Phage Φ-X174 was sequenced in 1977,[17] the DNA sequences of thousands of organisms have been decoded and stored in databases. This sequence information is analyzed to determine genes that encode proteins, RNA genes, regulatory sequences, structural motifs, and repetitive sequences. A comparison of genes within a species or between different species can show similarities between protein functions, or relations between species (the use of molecular systematics to construct phylogenetic trees). With the growing amount of data, it long ago became impractical to analyze DNA sequences manually. Today[when?]computer programssuch as BLAST are used daily to search sequences from more than 260 000 organisms, containing over 190 billion nucleotides.[18] These programs can compensate for mutations (exchanged, deleted or inserted bases) in the DNA sequence, to identify sequences that are related, but not identical. A variant of this sequence alignment is used in the sequencing process itself. For the special task of taxonomic classification of sequence snippets, modern k-mer based software like Kraken achieves throughput unreachable by alignment methods.

DNA sequencing[edit]

Before sequences can be analyzed they have to be obtained from the data storage bank example the Genbank. DNA sequencing is still a non-trivial problem as the raw data may be noisy or afflicted by weak signals. Algorithms have been developed for base calling for the various experimental approaches to DNA sequencing.

Sequence assembly[edit]

Most DNA sequencing techniques produce short fragments of sequence that need to be assembled to obtain complete gene or genome sequences. The so-called shotgun sequencing technique (which was used, for example, by The Institute for Genomic Research (TIGR) to sequence the first bacterial genome, Haemophilus influenzae)[19] generates the sequences of many thousands of small DNA fragments (ranging from 35 to 900 nucleotides long, depending on the sequencing technology). The ends of these fragments overlap and, when aligned properly by a genome assembly program, can be used to reconstruct the complete genome. Shotgun sequencing yields sequence data quickly, but the task of assembling the fragments can be quite complicated for larger genomes. For a genome as large as the human genome, it may take many days of CPU time on large-memory, multiprocessor computers to assemble the fragments, and the resulting assembly usually contains numerous gaps that must be filled in later. Shotgun sequencing is the method of choice for virtually all genomes sequenced today[when?], and genome assembly algorithms are a critical area of bioinformatics research.

Genome annotation[edit]

In the context of genomicsannotation is the process of marking the genes and other biological features in a DNA sequence. This process needs to be automated because most genomes are too large to annotate by hand, not to mention the desire to annotate as many genomes as possible, as the rate of sequencing has ceased to pose a bottleneck. Annotation is made possible by the fact that genes have recognisable start and stop regions, although the exact sequence found in these regions can vary between genes.
The first description of a comprehensive genome annotation system was published in 1995 [19] by the team at The Institute for Genomic Research that performed the first complete sequencing and analysis of the genome of a free-living organism, the bacterium Haemophilus influenzae.[19] Owen White designed and built a software system to identify the genes encoding all proteins, transfer RNAs, ribosomal RNAs (and other sites) and to make initial functional assignments. Most current genome annotation systems work similarly, but the programs available for analysis of genomic DNA, such as the GeneMark program trained and used to find protein-coding genes in Haemophilus influenzae, are constantly changing and improving.
Following the goals that the Human Genome Project left to achieve after its closure in 2003, a new project developed by the National Human Genome Research Institute in the U.S appeared. The so-called ENCODE project is a collaborative data collection of the functional elements of the human genome that uses next-generation DNA-sequencing technologies and genomic tiling arrays, technologies able to automatically generate large amounts of data at a dramatically reduced per-base cost but with the same accuracy (base call error) and fidelity (assembly error).

Computational evolutionary biology[edit]

Evolutionary biology is the study of the origin and descent of species, as well as their change over time. Informatics has assisted evolutionary biologists by enabling researchers to:
  • trace the evolution of a large number of organisms by measuring changes in their DNA, rather than through physical taxonomy or physiological observations alone,
  • compare entire genomes, which permits the study of more complex evolutionary events, such as gene duplicationhorizontal gene transfer, and the prediction of factors important in bacterial speciation,
  • build complex computational population genetics models to predict the outcome of the system over time[20]
  • track and share information on an increasingly large number of species and organisms
Future work endeavours to reconstruct the now more complex tree of life.
The area of research within computer science that uses genetic algorithms is sometimes confused with computational evolutionary biology, but the two areas are not necessarily related.

Comparative genomics[edit]

The core of comparative genome analysis is the establishment of the correspondence between genes (orthology analysis) or other genomic features in different organisms. It is these intergenomic maps that make it possible to trace the evolutionary processes responsible for the divergence of two genomes. A multitude of evolutionary events acting at various organizational levels shape genome evolution. At the lowest level, point mutations affect individual nucleotides. At a higher level, large chromosomal segments undergo duplication, lateral transfer, inversion, transposition, deletion and insertion.[21] Ultimately, whole genomes are involved in processes of hybridization, polyploidization and endosymbiosis, often leading to rapid speciation. The complexity of genome evolution poses many exciting challenges to developers of mathematical models and algorithms, who have recourse to a spectrum of algorithmic, statistical and mathematical techniques, ranging from exact, heuristics, fixed parameter and approximation algorithms for problems based on parsimony models to Markov chain Monte Carlo algorithms for Bayesian analysis of problems based on probabilistic models.
Many of these studies are based on the detection of sequence homology to assign sequences to protein families.[22]

Pan genomics[edit]

Pan genomics is a concept introduced in 2005 by Tettelin and Medini which eventually took root in bioinformatics. Pan genome is the complete gene repertoire of a particular taxonomic group: although initially applied to closely related strains of a species, it can be applied to a larger context like genus, phylum etc. It is divided in two parts- The Core genome: Set of genes common to all the genomes under study (These are often housekeeping genes vital for survival) and The Dispensable/Flexible Genome: Set of genes not present in all but one or some genomes under study. A bioinformatics tool BPGA can be used to characterize the Pan Genome of bacterial species.[23]

Genetics of disease[edit]

With the advent of next-generation sequencing we are obtaining enough sequence data to map the genes of complex diseases infertility,[24] breast cancer[25] or Alzheimer's disease.[26] Genome-wide association studies are a useful approach to pinpoint the mutations responsible for such complex diseases.[27] Through these studies, thousands of DNA variants have been identified that are associated with similar diseases and traits.[28] Furthermore, the possibility for genes to be used at prognosis, diagnosis or treatment is one of the most essential applications. Many studies are discussing both the promising ways to choose the genes to be used and the problems and pitfalls of using genes to predict disease presence or prognosis.[29]

Analysis of mutations in cancer[edit]

In cancer, the genomes of affected cells are rearranged in complex or even unpredictable ways. Massive sequencing efforts are used to identify previously unknown point mutationsin a variety of genes in cancer. Bioinformaticians continue to produce specialized automated systems to manage the sheer volume of sequence data produced, and they create new algorithms and software to compare the sequencing results to the growing collection of human genome sequences and germline polymorphisms. New physical detection technologies are employed, such as oligonucleotide microarrays to identify chromosomal gains and losses (called comparative genomic hybridization), and single-nucleotide polymorphism arrays to detect known point mutations. These detection methods simultaneously measure several hundred thousand sites throughout the genome, and when used in high-throughput to measure thousands of samples, generate terabytes of data per experiment. Again the massive amounts and new types of data generate new opportunities for bioinformaticians. The data is often found to contain considerable variability, or noise, and thus Hidden Markov model and change-point analysis methods are being developed to infer real copy number changes.
Two important principles can be used in the analysis of cancer genomes bioinformatically pertaining to the identification of mutations in the exome. First, cancer is a disease of accumulated somatic mutations in genes. Second cancer contains driver mutations which need to be distinguished from passengers.[30]
With the breakthroughs that this next-generation sequencing technology is providing to the field of Bioinformatics, cancer genomics could drastically change. These new methods and software allow bioinformaticians to sequence many cancer genomes quickly and affordably. This could create a more flexible process for classifying types of cancer by analysis of cancer driven mutations in the genome. Furthermore, tracking of patients while the disease progresses may be possible in the future with the sequence of cancer samples.[31]
Another type of data that requires novel informatics development is the analysis of lesions found to be recurrent among many tumors.

Gene and protein expression[edit]

Analysis of gene expression[edit]

The expression of many genes can be determined by measuring mRNA levels with multiple techniques including microarraysexpressed cDNA sequence tag (EST) sequencing, serial analysis of gene expression (SAGE) tag sequencing, massively parallel signature sequencing (MPSS), RNA-Seq, also known as "Whole Transcriptome Shotgun Sequencing" (WTSS), or various applications of multiplexed in-situ hybridization. All of these techniques are extremely noise-prone and/or subject to bias in the biological measurement, and a major research area in computational biology involves developing statistical tools to separate signal from noise in high-throughput gene expression studies.[32] Such studies are often used to determine the genes implicated in a disorder: one might compare microarray data from cancerous epithelial cells to data from non-cancerous cells to determine the transcripts that are up-regulated and down-regulated in a particular population of cancer cells.

Analysis of protein expression[edit]

Protein microarrays and high throughput (HT) mass spectrometry (MS) can provide a snapshot of the proteins present in a biological sample. Bioinformatics is very much involved in making sense of protein microarray and HT MS data; the former approach faces similar problems as with microarrays targeted at mRNA, the latter involves the problem of matching large amounts of mass data against predicted masses from protein sequence databases, and the complicated statistical analysis of samples where multiple, but incomplete peptides from each protein are detected. Cellular protein localization in a tissue context can be achieved through affinity proteomics displayed as spatial data based on immunohistochemistryand tissue microarrays.[33]

Analysis of regulation[edit]

Gene regulation is the complex orchestration of events by which a signal, potentially an extracellular signal such as a hormone, eventually leads to an increase or decrease in the activity of one or more proteins. Bioinformatics techniques have been applied to explore various steps in this process.
For example, gene expression can be regulated by nearby elements in the genome. Promoter analysis involves the identification and study of sequence motifs in the DNA surrounding the coding region of a gene. These motifs influence the extent to which that region is transcribed into mRNA. Enhancer elements far away from the promoter can also regulate gene expression, through three-dimensional looping interactions. These interactions can be determined by bioinformatic analysis of chromosome conformation captureexperiments.
Expression data can be used to infer gene regulation: one might compare microarray data from a wide variety of states of an organism to form hypotheses about the genes involved in each state. In a single-cell organism, one might compare stages of the cell cycle, along with various stress conditions (heat shock, starvation, etc.). One can then apply clustering algorithms to that expression data to determine which genes are co-expressed. For example, the upstream regions (promoters) of co-expressed genes can be searched for over-represented regulatory elements. Examples of clustering algorithms applied in gene clustering are k-means clusteringself-organizing maps (SOMs), hierarchical clustering, and consensus clustering methods.

Analysis of cellular organization[edit]

Several approaches have been developed to analyze the location of organelles, genes, proteins, and other components within cells. This is relevant as the location of these components affects the events within a cell and thus helps us to predict the behavior of biological systems. A gene ontology category, cellular compartment, has been devised to capture subcellular localization in many biological databases.

Microscopy and image analysis[edit]

Microscopic pictures allow us to locate both organelles as well as molecules. It may also help us to distinguish between normal and abnormal cells, e.g. in cancer.

Protein localization[edit]

The localization of proteins helps us to evaluate the role of a protein. For instance, if a protein is found in the nucleus it may be involved in gene regulation or splicing. By contrast, if a protein is found in mitochondria, it may be involved in respiration or other metabolic processes. Protein localization is thus an important component of protein function prediction. There are well developed protein subcellular localization prediction resources available, including protein subcellular location databases, and prediction tools.[34][35]

Nuclear organization of chromatin[edit]

Data from high-throughput chromosome conformation capture experiments, such as Hi-C (experiment) and ChIA-PET, can provide information on the spatial proximity of DNA loci. Analysis of these experiments can determine the three-dimensional structure and nuclear organization of chromatin. Bioinformatic challenges in this field include partitioning the genome into domains, such as Topologically Associating Domains (TADs), that are organised together in three-dimensional space.[36]

Structural bioinformatics[edit]


3-dimensional protein structures such as this one are common subjects in bioinformatic analyses.
Protein structure prediction is another important application of bioinformatics. The amino acid sequence of a protein, the so-called primary structure, can be easily determined from the sequence on the gene that codes for it. In the vast majority of cases, this primary structure uniquely determines a structure in its native environment. (Of course, there are exceptions, such as the bovine spongiform encephalopathy(mad cow disease) prion.) Knowledge of this structure is vital in understanding the function of the protein. Structural information is usually classified as one of secondarytertiary and quaternary structure. A viable general solution to such predictions remains an open problem. Most efforts have so far been directed towards heuristics that work most of the time.[citation needed]
One of the key ideas in bioinformatics is the notion of homology. In the genomic branch of bioinformatics, homology is used to predict the function of a gene: if the sequence of gene A, whose function is known, is homologous to the sequence of gene B, whose function is unknown, one could infer that B may share A's function. In the structural branch of bioinformatics, homology is used to determine which parts of a protein are important in structure formation and interaction with other proteins. In a technique called homology modeling, this information is used to predict the structure of a protein once the structure of a homologous protein is known. This currently remains the only way to predict protein structures reliably.
One example of this is hemoglobin in humans and the hemoglobin in legumes (leghemoglobin), which are distant relatives from the same protein superfamily. Both serve the same purpose of transporting oxygen in the organism. Although both of these proteins have completely different amino acid sequences, their protein structures are virtually identical, which reflects their near identical purposes and shared ancestor.[37]
Other techniques for predicting protein structure include protein threading and de novo (from scratch) physics-based modeling.
Another aspect of structural bioinformatics include the use of protein structures for Virtual Screening models such as Quantitative Structure-Activity Relationship models and proteochemometric models (PCM). Furthermore, a protein's crystal structure can be used in simulation of for example ligand-binding studies and in silico mutagenesis studies.

Network and systems biology[edit]

Network analysis seeks to understand the relationships within biological networks such as metabolic or protein–protein interaction networks. Although biological networks can be constructed from a single type of molecule or entity (such as genes), network biology often attempts to integrate many different data types, such as proteins, small molecules, gene expression data, and others, which are all connected physically, functionally, or both.
Systems biology involves the use of computer simulations of cellular subsystems (such as the networks of metabolites and enzymes that comprise metabolismsignal transductionpathways and gene regulatory networks) to both analyze and visualize the complex connections of these cellular processes. Artificial life or virtual evolution attempts to understand evolutionary processes via the computer simulation of simple (artificial) life forms.

Molecular interaction networks[edit]


Interactions between proteins are frequently visualized and analyzed using networks. This network is made up of protein–protein interactions from Treponema pallidum, the causative agent of syphilis and other diseases.
Tens of thousands of three-dimensional protein structures have been determined by X-ray crystallography and protein nuclear magnetic resonance spectroscopy (protein NMR) and a central question in structural bioinformatics is whether it is practical to predict possible protein–protein interactions only based on these 3D shapes, without performing protein–protein interaction experiments. A variety of methods have been developed to tackle the protein–protein docking problem, though it seems that there is still much work to be done in this field.
Other interactions encountered in the field include Protein–ligand (including drug) and protein–peptide. Molecular dynamic simulation of movement of atoms about rotatable bonds is the fundamental principle behind computational algorithms, termed docking algorithms, for studying molecular interactions.

Others[edit]

Literature analysis[edit]

The growth in the number of published literature makes it virtually impossible to read every paper, resulting in disjointed sub-fields of research. Literature analysis aims to employ computational and statistical linguistics to mine this growing library of text resources. For example:
  • Abbreviation recognition – identify the long-form and abbreviation of biological terms
  • Named entity recognition – recognizing biological terms such as gene names
  • Protein–protein interaction – identify which proteins interact with which proteins from text
The area of research draws from statistics and computational linguistics.

High-throughput image analysis[edit]

Computational technologies are used to accelerate or fully automate the processing, quantification and analysis of large amounts of high-information-content biomedical imagery. Modern image analysis systems augment an observer's ability to make measurements from a large or complex set of images, by improving accuracyobjectivity, or speed. A fully developed analysis system may completely replace the observer. Although these systems are not unique to biomedical imagery, biomedical imaging is becoming more important for both diagnostics and research. Some examples are:
  • high-throughput and high-fidelity quantification and sub-cellular localization (high-content screening, cytohistopathology, Bioimage informatics)
  • morphometrics
  • clinical image analysis and visualization
  • determining the real-time air-flow patterns in breathing lungs of living animals
  • quantifying occlusion size in real-time imagery from the development of and recovery during arterial injury
  • making behavioral observations from extended video recordings of laboratory animals
  • infrared measurements for metabolic activity determination
  • inferring clone overlaps in DNA mapping, e.g. the Sulston score

High-throughput single cell data analysis[edit]

Computational techniques are used to analyse high-throughput, low-measurement single cell data, such as that obtained from flow cytometry. These methods typically involve finding populations of cells that are relevant to a particular disease state or experimental condition.

Biodiversity informatics[edit]

Biodiversity informatics deals with the collection and analysis of biodiversity data, such as taxonomic databases, or microbiome data. Examples of such analyses include phylogeneticsniche modellingspecies richness mapping, DNA barcoding, or species identification tools.

Ontologies and data integration[edit]

Biological ontologies are directed acyclic graphs of controlled vocabularies. They are designed to capture biological concepts and descriptions in a way that can be easily categorised and analysed with computers. When categorised in this way, it is possible to gain added value from holistic and integrated analysis.
The OBO Foundry was an effort to standardise certain ontologies. One of the most widespread is the Gene ontology which describes gene function. There are also ontologies which describe phenotypes.

Databases[edit]

Databases are essential for bioinformatics research and applications. Many databases exist, covering various information types: for example, DNA and protein sequences, molecular structures, phenotypes and biodiversity. Databases may contain empirical data (obtained directly from experiments), predicted data (obtained from analysis), or, most commonly, both. They may be specific to a particular organism, pathway or molecule of interest. Alternatively, they can incorporate data compiled from multiple other databases. These databases vary in their format, access mechanism, and whether they are public or not.
Some of the most commonly used databases are listed below. For a more comprehensive list, please check the link at the beginning of the subsection.

Software and tools[edit]

Software tools for bioinformatics range from simple command-line tools, to more complex graphical programs and standalone web-services available from various bioinformatics companies or public institutions.

Open-source bioinformatics software[edit]

Many free and open-source software tools have existed and continued to grow since the 1980s.[38] The combination of a continued need for new algorithms for the analysis of emerging types of biological readouts, the potential for innovative in silico experiments, and freely available open code bases have helped to create opportunities for all research groups to contribute to both bioinformatics and the range of open-source software available, regardless of their funding arrangements. The open source tools often act as incubators of ideas, or community-supported plug-ins in commercial applications. They may also provide de facto standards and shared object models for assisting with the challenge of bioinformation integration.
The range of open-source software packages includes titles such as BioconductorBioPerlBiopythonBioJavaBioJSBioRubyBioclipseEMBOSS.NET BioOrange with its bioinformatics add-on, Apache TavernaUGENE and GenoCAD. To maintain this tradition and create further opportunities, the non-profit Open Bioinformatics Foundation[38] have supported the annual Bioinformatics Open Source Conference (BOSC) since 2000.[39]
An alternative method to build public bioinformatics databases is to use the MediaWiki engine with the WikiOpener extension. This system allows the database to be accessed and updated by all experts in the field.[40]

Web services in bioinformatics[edit]

SOAP- and REST-based interfaces have been developed for a wide variety of bioinformatics applications allowing an application running on one computer in one part of the world to use algorithms, data and computing resources on servers in other parts of the world. The main advantages derive from the fact that end users do not have to deal with software and database maintenance overheads.
Basic bioinformatics services are classified by the EBI into three categories: SSS (Sequence Search Services), MSA (Multiple Sequence Alignment), and BSA (Biological Sequence Analysis).[41] The availability of these service-oriented bioinformatics resources demonstrate the applicability of web-based bioinformatics solutions, and range from a collection of standalone tools with a common data format under a single, standalone or web-based interface, to integrative, distributed and extensible bioinformatics workflow management systems.

Bioinformatics workflow management systems[edit]

bioinformatics workflow management system is a specialized form of a workflow management system designed specifically to compose and execute a series of computational or data manipulation steps, or a workflow, in a Bioinformatics application. Such systems are designed to
  • provide an easy-to-use environment for individual application scientists themselves to create their own workflows,
  • provide interactive tools for the scientists enabling them to execute their workflows and view their results in real-time,
  • simplify the process of sharing and reusing workflows between the scientists, and
  • enable scientists to track the provenance of the workflow execution results and the workflow creation steps.
Some of the platforms giving this service: GalaxyKeplerTavernaUGENEAndurilHIVE.

BioCompute and BioCompute Objects[edit]

In 2014, the US Food and Drug Administration sponsored a conference held at the National Institutes of Health Bethesda Campus to discuss reproducibility in bioinformatics.[42] Over the next three years, a consortium of stakeholders met regularly to discuss what would become BioCompute paradigm.[43] These stakeholders included representatives from government, industry, and academic entities. Session leaders represented numerous branches of the FDA and NIH Institutes and Centers, non-profit entities including the Human Variome Project and the European Federation for Medical Informatics, and research institutions including Stanford, the New York Genome Center, and the George Washington University.
It was decided that the BioCompute paradigm would be in the form of digital ‘lab notebooks’ which allow for the reproducibility, replication, review, and reuse, of bioinformatics protocols. This was proposed to enable greater continuity within a research group over the course of normal personnel flux while furthering the exchange of ideas between groups. The US FDA funded this work so that information on pipelines would be more transparent and accessible to their regulatory staff.[44]
In 2016, the group reconvened at the NIH in Bethesda and discussed the potential for a BioCompute Object, an instance of the BioCompute paradigm. This work was copied as both a “standard trial use” document and a preprint paper uploaded to bioRxiv. The BioCompute object allows for the JSON-ized record to be shared among employees, collaborators, and regulators.[45][46]

Education platforms[edit]

Software platforms designed to teach bioinformatics concepts and methods include Rosalind and online courses offered through the Swiss Institute of Bioinformatics Training Portal. The Canadian Bioinformatics Workshops provides videos and slides from training workshops on their website under a Creative Commons license. The 4273Ï€ project or 4273pi project[47] also offers open source educational materials for free. The course runs on low cost Raspberry Pi computers and has been used to teach adults and school pupils.[48][49]4273Ï€ is actively developed by a consortium of academics and research staff who have run research level bioinformatics using Raspberry Pi computers and the 4273Ï€ operating system.[50][51]
MOOC platforms also provide online certifications in bioinformatics and related disciplines, including Coursera's Bioinformatics Specialization (UC San Diego) and Genomic Data Science Specialization (Johns Hopkins) as well as EdX's Data Analysis for Life Sciences XSeries (Harvard). University of Southern California offers a Masters In Translational Bioinformatics focusing on biomedical applications.

Conferences[edit]

There are several large conferences that are concerned with bioinformatics. Some of the most notable examples are Intelligent Systems for Molecular Biology (ISMB), European Conference on Computational Biology (ECCB), and Research in Computational Molecular Biology (RECOMB).



Bioinformatics

TABLE OF CONTENTS
  • Introduction
  • The data of bioinformatics
  • Storage and retrieval of data
  • Goals of bioinformatics


Bioinformatics, a hybrid science that links biological data with techniques for information storage, distribution, and analysis to support multiple areas of scientific research, including biomedicine. Bioinformatics is fed by high-throughput data-generating experiments, including genomic sequence determinations and measurements of gene expression patterns. Database projects curate and annotate the data and then distribute it via the World Wide Web. Mining these data leads to scientific discoveries and to the identification of new clinical applications. In the field of medicine in particular, a number of important applications for bioinformatics have been discovered. For example, it is used to identify correlations between gene sequences and diseases, to predict protein structures from amino acid sequences, to aid in the design of novel drugs, and to tailor treatments to individual patients based on their DNA sequences (pharmacogenomics).


The data of bioinformatics

The classic data of bioinformatics include DNA sequences of genes or full genomes; amino acid sequences of proteins; and three-dimensional structures of proteins, nucleic acids and protein–nucleic acid complexes. Additional “-omics” data streams include: transcriptomics, the pattern of RNA synthesis from DNA; proteomics, the distribution of proteins in cellsinteractomics, the patterns of protein-protein and protein–nucleic acid interactions; and metabolomics, the nature and traffic patterns of transformations of small molecules by the biochemical pathways active in cells. In each case there is interest in obtaining comprehensive, accurate data for particular cell types and in identifying patterns of variation within the data. For example, data may fluctuate depending on cell type, timing of data collection (during the cell cycle, or diurnal, seasonal, or annual variations), developmental stage, and various external conditions. Metagenomics and metaproteomics extend these measurements to a comprehensive description of the organisms in an environmental sample, such as in a bucket of ocean water or in a soil sample.
Bioinformatics has been driven by the great acceleration in data-generation processes in biology. Genome sequencing methods show perhaps the most dramatic effects. In 1999 the nucleic acid sequence archives contained a total of 3.5 billion nucleotides, slightly more than the length of a single human genome; a decade later they contained more than 283 billion nucleotides, the length of about 95 human genomes. The U.S. National Institutes of Health has challenged researchers by setting a goal to reduce the cost of sequencing a human genome to $1,000; this would make DNA sequencing a more affordable and practical tool for U.S. hospitals and clinics, enabling it to become a standard component of diagnosis.

Storage and retrieval of data

In bioinformatics, data banks are used to store and organize data. Many of these entities collect DNA and RNA sequences from scientific papers and genome projects. Many databases are in the hands of international consortia. For example, an advisory committee made up of members of the European Molecular Biology Laboratory Nucleotide Sequence Database (EMBL-Bank) in the United Kingdom, the DNA Data Bank of Japan (DDBJ), and GenBank of the National Center for Biotechnology Information (NCBI) in the United States oversees the International Nucleotide Sequence Database Collaboration (INSDC). To ensure that sequence data are freely available, scientific journals require that new nucleotide sequences be deposited in a publicly accessible database as a condition for publication of an article. (Similar conditions apply to nucleic acid and protein structures.) There also exist genome browsers, databases that bring together all the available genomic and molecular information about a particular species.
The major database of biological macromolecular structure is the worldwide Protein Data Bank (wwPDB), a joint effort of the Research Collaboratory for Structural Bioinformatics (RCSB) in the United States, the Protein Data Bank Europe (PDBe) at the European Bioinformatics Institute in the United Kingdom, and the Protein Data Bank Japan at ÅŒsaka University. The homepages of the wwPDB partners contain links to the data files themselves, to expository and tutorial material (including news items), to facilities for deposition of new entries, and to specialized search software for retrieving structures.
Information retrieval from the data archives utilizes standard tools for identification of data items by keyword; for instance, one can type “aardvark myoglobin” into Google and retrieve the molecule’s amino acid sequence. Other algorithms search data banks to detect similarities between data items. For example, a standard problem is to probe a sequence database with a gene or protein sequence of interest in order to detect entities with similar sequences.

Goals of bioinformatics

The development of efficient algorithms for measuring sequence similarity is an important goal of bioinformatics. The Needleman-Wunsch algorithm, which is based on dynamic programming, guarantees finding the optimal alignment of pairs of sequences. This algorithm essentially divides a large problem (the full sequence) into a series of smaller problems (short sequence segments) and uses the solutions of the smaller problems to construct a solution to the large problem. Similarities in sequences are scored in a matrix, and the algorithm allows for the detection of gaps in sequence alignment.
Although the Needleman-Wunsch algorithm is effective, it is too slow for probing a large sequence database. Therefore, much attention has been given to finding fast information-retrieval algorithms that can deal with the vast amounts of data in the archives. An example is the program BLAST (Basic Local Alignment Search Tool). A development of BLAST, known as position-specific iterated- (or PSI-) BLAST, makes use of patterns of conservation in related sequences and combines the high speed of BLAST with very high sensitivity to find related sequences.
Another goal of bioinformatics is the extension of experimental data by predictions. A fundamental goal of computational biology is the prediction of protein structure from an amino acid sequence. The spontaneous folding of proteins shows that this should be possible. Progress in the development of methods to predict protein folding is measured by biennial Critical Assessment of Structure Prediction (CASP) programs, which involve blind tests of structure prediction methods.
Bioinformatics is also used to predict interactions between proteins, given individual structures of the partners. This is known as the “docking problem.” Protein-protein complexes show good complementarity in surface shape and polarity and are stabilized largely by weak interactions, such as burial of hydrophobic surface, hydrogen bonds, and van der Waals forces. Computer programs simulate these interactions to predict the optimal spatial relationship between binding partners. A particular challenge, one that could have important therapeutic applications, is to design an antibody that binds with high affinity to a target protein.
Initially, much bioinformatics research has had a relatively narrow focus, concentrating on devising algorithms for analyzing particular types of data, such as gene sequences or protein structures. Now, however, the goals of bioinformatics are integrative and are aimed at figuring out how combinations of different types of data can be used to understand natural phenomena, including organisms and disease.
Arthur M. Lesk
 
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