ஓம் ரவிசுதாய வித்மஹே மந்தக்ரஹாய தீமஹி தந்நோ சனி ப்ரஜோதயாத்; ஓம் காகத்வஜாய வித்மஹே கஹட்கஹஸ்தாய தீமஹி தந்நோ சனி ப்ரஜோதயாத்; ஓம் சதுர்புஜாய வித்மஹே தண்டஹஸ்தாய தீமஹி தந்நோ மந்தஹ் ப்ரஜோதயாத்; ஓம் சனீஸ்வராய வித்மஹே சாய புத்ராய தீமஹி தந்நோ சனி ப்ரஜோதயாத்; நீலாஞ்சனம் சமாபாஷம் ரவிபுத்ரம் எமாக்ரஜம் சாய மார்தாண்ட சம்பூதம் தம்நமாமி சனிஷ் ச்சரம்




Ultraviolet-Visible (UV-VIS) Spectroscopy is an analytical technique that can quantitate the amount of analyte based on the amount of light the analyte absorbs.  If the analyte in solution is colored, analysis in the visible spectrum is typically used.  This is because the physics involved with color.  Visible light is called visible light because when different wavelengths of light are separated (by a prism for example), the light can visualized as a color.  Light travels as photons and photons move in waves. The wavelengths of light that can be visualized by humans are tabulated below.
Color of LightWavelengthComplimentary Color
Violet390-450 nmYellow
Blue450-495 nmOrange
Green495-570 nmRed
Yellow570-590 nmViolet
Orange590-620 nmBlue
Red620-750 nmGreen

Complementary colors are also important when discussing the perceived color of objects. If the object reflects all colors of light but absorbs yellow, the eye will perceive the object as violet which is the complementary color yellow. The color of an object is determined by what wavelengths of light the object absorbs and reflections.
Now back to UV-VIS spectroscopy. If a solution of red liquid is placed in a cuvette and a visible light source shines on that red liquid, the detector on the other side the cuvette will show that the red solution reflects all color of light, but it absorbs green light, the complementary color to red.  Therefore, the absorbance peak of a red solution generated by the spectrophotometer will be in the green wavelength range.
To humans, light in the UV range is not perceived as color.  Other animals can perceive UV light as color, but humans cannot.  To us humans, solutions that absorb UV light appear to be clear. However, many different molecules can absorb light in the UV range.  For example, DNA is known to absorb light at 260nm and most proteins can absorb light at 280nm. UV/VIS spectroscopy is the most common type of detector for HPLC.  The spectrum and retention time can provide useful information regarding identity and purity of compounds.


 
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